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KMID : 1007520230320142079
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Food Science and Biotechnology
2023 Volume.32 No. 14 p.2079 ~ p.2092
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Pleurotus florida mediated biosynthesis of nanoparticles and biofortification
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Shivnam Rana
Shammi Kapoor
Yoon Hye-Young
Anu Kalia
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Abstract
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The mycosynthesis of biogenic NPs using nanotechnology technique is an ecofriendly and economical approach. The extracellular mycelial extract of the Pleurotus florida fungi were used to biosynthesized Zn, Cu and Fe NPs using zinc sulphate, zinc chloride, copper sulphate, copper chloride ferrous sulphate and ferric chloride, precursor salts at 1.0 mM concentration. The color of reaction mixture was changed from (transparent to white, blue to green and yellow to brown) for Zn, Cu and Fe NPs during incubation period of 96 h at 25?±?2 °C, indicating synthesis of NPs. Spectroscopy and microscopy techniques were used for the characterization of newly synthesized biogenic NPs. Whereas, the ICP-MS analysis revealed that copper chloride precursor salts produced high concentration of Cu biogenic NPs, followed by zinc chloride derived Zn NPs. The fortification with the biogenic NPs of Pleurotus florida mycelium exhibited high accumulation of the trace elements as compared to non-fortified mycelium.
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KEYWORD
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Biosynthesis, Bioaccumulation, Biogenic NPs, Characterization, P. florida
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